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KRAS and BRAF mutations predict the resistance of colorectal carcinomas to therapy targeted to the epidermal growth factor receptor, but their detection can be challenging because of high testing volume, frequently low tumor content, and the spectrum of rarer mutations in these genes.

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Sep 09, 2015 · Tiacci and colleagues found that the V600E mutation of BRAF, a kinase commonly mutated in solid tumors, 9 was the key genetic lesion of hairy-cell leukemia, 10,11 and Chung et al. found that this

Aim 2 concerns the study of Class 3 BRAF mutants, the most common class in lung cancer, and exploits a novel mechanism for inhibiting their activity. Aim 3 concerns the preclinical development of the KRAS G12C inhibitor and its use to study the functional role of mutant RAS in lung cancer and the consequences of its inhibition. Inhibiting ERK Dec 06, 2019 · Testing methodology was specified in the majority of cases (79% for KRAS, 92% NRAS, 87% BRAF, and 94% MSI/dMMR). Although PCR was the most common testing methodology for KRAS in 2013, NGS was the dominant testing methodology for KRAS after 2013 and for NRAS and BRAF throughout the entire period of this study. A history or evidence of current ≥ class II congestive heart failure as defined by the New York Heart Association (NYHA) guidelines (Appendix 4: New York Heart Association (NYHA) Guidelines); f. Treatment refractory hypertension defined as a blood pressure of systolic >140 mmHg and/or diastolic > 90 mm Hg which cannot be controlled by May 31, 2016 · Prior confirmation of tumour “quadruple wild type” status – ie.no mutations in KRAS and NRAS (exons 2, 3, 4), BRAF exon 15, and PIK3CA exons 9 and 20 or KRAS G13D mutant, by means of mutation or relevant analysis performed on representative samples of diagnostic tumour tissue

Mutation of KRAS is a common initiating event in pancreatic ductal adenocarcinoma (PDAC). Yet, the specific roles of KRAS-stimulated signaling pathways in the transformation of pancreatic ductal epithelial cells (PDEC), putative cells of origin for PDAC, remain unclear. Here, we show that KRASG12D and BRAFV600E enhance PDEC proliferation and increase survival after exposure to apoptotic

"V600E BRAF had an at least three fold stronger impact on overall and disease-free survival compared to KRAS mutation and was the most powerful prognostic determinant in this multi-institutional cohort," said Dr. Margonis. Nevertheless, he and his colleagues stress that further studies are needed.

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Loupakis F, Ruzzo A, Cremolini C, et al. KRAS codon 61, 146 and BRAF mutations predict resistance to cetuximab plus irinotecan in KRAS codon 12 and 13 wild-type metastatic colorectal cancer. Br J Dec 17, 2012 · Part 1, Part 2 and BRAF-mutant patients in Part 4 ONLY: Known presence of KRAS-mutation based on previous KRAS-testing. Note: Propsective KRAS testing is not required. However, if the results of previous KRAS testing are known, they must be used in assessing eligibility. KRAS testing will be performed retrospectively for all patients.